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The main web application on port 80 was running on sysadmin account in application. I changed identity to Domain user account. Added that user to config and admin databases as db owner and securityadmin.

From that onward website is displaying

Cannot connect to the configuration database.

I added domain user into farm admin group also. Still did not worked.

I changed back to sysadmin account and working fine. But I don't want use sysadmin account.

  • How you change the app pool account, from sharepoint central admin or manaully on all server?e – Waqas Sarwar MVP Jan 5 '15 at 20:48
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Here are the best practice to change the app Pool account.

  • First add new account into the Managed Account( security > configure managed Accounts)
  • Make sure new account Have DBO rights on the Config DB & Content Databasea.
  • from Central Admin > security > Configure Service Account...select the App pool and from next drop down select the account. Click OK.
  • Now Perform IIS reset on all servers in farm.

http://nikpatel.net/2012/01/25/best-practices-to-change-app-pool-account-for-sharepoint-web-applcation/

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