I have created a new web application with its own application pool, and created a new site collection, setting both a primary and secondary site collection administrator.
When I try to browse the site with one of these accounts, I get 'Access denied'. The page returned is
Browsing the site in Sharepoint Designer with the site collection adminstrator, I am unable to view default.aspx - I get the error
'You do not have permission to do this operation. Ask your web site administrator to change your permissions and then try again, or log in with a user account that has this permission. To log on with a different account click OK.'
I am unable to access the site both from the SharePoint server and from other machines.
I have tried adding the users to the Policy for Web Application screen, with Full Control and Full Read rights. I have tried removing the web application and recreating it.
The SharePoint farm (one server) currently hosts 22 web applications (including this one), I don't have any issues with any of the other sites.