I have numerous js css, and custom pages deployed to the _layouts folder of my SharePoint 2007 farm. These are used by numerous farm wide solutions. Today, something has gone wrong with the permissions on the _layout folder on the one server and we are getting "http 404 not found" errors when trying to access these files.
I have compared the security on this server to the other servers in the farm and they are the same. Has anyone had this before?